The first generation of ElectroCapture Instruments™ has been designed for the analysis of peptides and proteins by mass spectrometry. It is entirely based on the ElectroCapture Cell™ technology which was developed searching for simple, more versatile strategies to perform sample preparation.
The first generation of ElectroCapture Instruments™ has been designed for the analysis of peptides and proteins by mass spectrometry. It is entirely based on the ElectroCapture Cell™ technology which was developed searching for simple, more versatile strategies to perform sample preparation.
The instrument is capable of carrying out key analytical steps performed on proteome samples - alkylation, trypsin digestion, concentration, sample cleanup and fractionation of polypeptides. The system, efficiently removing non-ionic detergents, represents a unique tool for the analysis (separation and cleanup) of membrane receptors and other membrane-associated proteins.
Low sample and reagents consumption, combined with simple, low cost maintenance make this microfluidic platform a perfect complement to liquid chromatography and other separation and sample preparation methods.
The ElectroCapture Instrument™ is an automated system platform, where only sample injection is performed manually (in the same manner as most HPLC systems).
The pump and power supply is software controlled, allowing programming for different settings of capture voltage and gradients.
The ECI™ can be delivered with an optional autosampler, Maldi spotter and fraction collector.
It has an open configuration for straightforward connection to any ESI-MS instrument using standard microbore tubing fittings.
The ElectroCapture Cell™ can be used for several months if proper cleaning and handling procedures are followed, and the replacement procedure is manual, simple and straight forward.
Sample volume after processing:~ 400nL
Separation: 1.5-2 hrs for 30 - 40 fractions.
Concentration: 10 µL to 400 nL in 20 min.
Microreactions: For reduction, alkylation and trypsin digestion: about 1.5 hrs for 1 pmol of protein.
Buffer exchange/Desalting: 30 min to 1 hrs.
Discover the exciting opportunity to have the latest multipurpose microfluidic technology in your lab. Please feel free to contact us for further information!
